Overview of Research

Introduction

“A unique data source to advance established research questions with greater depth and accuracy.”

1- A Unique Source of Data ……

  • Temporal resolution: Data can be collected across multiple timescales — within a day, across weeks, months, years, and over the entire lifespan of the dog.

  • High-frequency sampling: Daily fresh samples enable precise laboratory analyses, including cultivation, microscopy, DNA, protein, and RNA profiling.

  • Repeated measures: Because dogs are walked daily or weekly, repeated sampling is feasible, allowing correction, refinement, and improvement of analyses.

  • Rich metadata: Owners can provide highly accurate contextual information, including age, diet, social interactions, environment, and behavior.

2- To advanced Major Questions

  • How long can gut-dwelling organism live in the intestine?

  • How does the diet impact the gut microbiome?

Dogs on walks have very different diets — some eat mixed diets, while others eat only raw meat, fish, or beef. How does a dog’s diet, or changes in diet over time, affect the microbes living in their gut?

  • How does the environment impact the gut microbiome?

A few examples:

  • Are dogs that spend time in areas with lots of horses (like Southlands) more likely to pick up bacteria linked to horses?

  • Are dogs that live or play near the beach (like Spanish Banks Off-Leash Area or Hadden Beach) more likely to pick up bacteria from the sea?

  • Are dogs that live or play near fresh water (like Fraser River) more likely to pick up bacteria from fresh water

  • Are some gut organism associated with the dog health?

A few examples:

  • What is the effect of spaying or neutering on the gut microbiome of dogs?

  • Do dogs with allergies (like chicken allergies) have a different community of microbes in their gut?

  • How do antibiotics and other medications, including anti-parasitic drugs, affect the microbes living in the gut?

  • Does the abundance of gut microbe change over time ?

  • How gut microbe interact with each other?

Methods

Fecal sample collection

Sample storing

PCR, qPCR

Data Analysis

Microscopy

DNA extraction

Sequencing

Results

Discussion

References