• Temporal data within an individual. Catch and release (weeks, months, years)

• Temporal data. Season, years, within a day

• Social data. parents, genealogy, fight, social status, mating, behaviour

• Incomplete have individual biological metadata. Animals are not killed, so we do not have access to all organs.

• Large sample size. Many mice are inside the dome.

• Geographic data within a site. More like social spllting

QUESTIONS

Does interaction influence gut microbiome composition?

Does location influence gut microbiome composition?

How much does the gut microbiome change with an individual (over hours, days, weeks, months, years)?

Does season influence gut microbiome?

Does diet influence gut microbiome?

Does genetics influence the gut microbiome?

Does biological sex influence the gut microbiome?